Each sample was placed in the reactor and submersed in 500 700 ml of MB water solution (1.3 ± 0.07 μmol l-1), ensuring that the thickness of the solution layer above the catalytic surface was constant (12 ± 1 mm) for all measurements.
The brains were extracted and submersed in 20% sucrose in phosphate buffer overnight.
The samples were loaded by pneumatic grips and submersed in phosphate buffer solution (pre-heated to 37 ± 3 °C) in a BioPuls™ unit for 5 min prior to stretching.
The samples were loaded by pneumatic grips and submersed in phosphate buffer solution (pre-heated to 37°C ± 3°C) in a BioPuls™ unit for 5 min prior to stretching.
For VTA slices, the brain was then blocked to isolate the VTA before being glued onto the cutting stage of a vibrating tissue slicer (Leica, VT1000) and submersed in ice-cold, oxygenated aCSF.
Hybridization was carried out in a Gene Machines HybChamberTM humidified with 3× SSC and submersed in a 65°C water bath for 14 18 hours.
Therefore, encounters of aphids with different wasps as well as with aphidophagous larvae were first filmed and afterwards some exemplars of attacking organisms were caught and submersed in alcohol.
The system performance was validated by acquiring and analyzing OCT/FLIM images of capillary tubes filled with fluorophore solutions and submersed in a scattering medium.
The microarray slides were enclosed in ArrayIt (Telechem International) hybridization chambers and submersed in a heated water bath and incubated for 16-20 h at 42°C.
We consider the problem of localization and tracking for mobile units submersed in the ocean.
