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Then the growth medium was equilibrated with 1/10 VT of 100 mM Tris/HCl (pH 7.0) and subjected with a syringe to a HiTrap™ HP Heparin column (5 ml) that was equilibrated with 25 ml of 10 mM Tris/HCl (pH 7.0).
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The insomnia questions were then added, and subjects with a score ≥ 1 were considered likely to have insomnia.
These two answer scores were added, and subjects with a sum score of 0 were considered unlikely to have insomnia [24 26].
We excluded patients who had undergone pharmacological prophylactic treatment for headaches in the 6 months preceding the study and subjects with a frequency of headache lower than one every two months.
Multiple recent studies have used new whole-genome genotyping methods to discover structural variations in the DNA segments of normal subjects and subjects with a variety of disorders.
To minimize confounding by non-ALS-related alterations of the brain we excluded subjects older than 65 years of age and subjects with a history of brain injury, epilepsy, vascular risk factors, psychiatric illness and other systemic diseases.
The self-reporting of WC is especially less accurate in males, younger subjects and subjects with a lower measured WC.
Regression to the mean will be present when subjects with a poor physical health (lower quartile) improve and subjects with a good physical health (upper quartile) deteriorate likewise.
The chi-square test between subjects with a total delay < 20 weeks and subjects with a total delay ≥ 20 weeks resulted not significant for the variables considered.
Weighted mean HbGA concentrations were higher in young adults, active smokers, and subjects with a lower education level.
Routine systemic medications used by the subjects were documented and subjects with a change in the systemic medications during the study period would be excluded.
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