Sentence examples for and stopped with a from inspiring English sources

At that moment, the red pickup sped back into sight, fifty yards down the slope from us, and stopped with a lurch.

There are BMXs, often fitted with a freewheel and stopped with a trainer to the tyre, the braver subsection of fixed-gear riders and those whose bike is badly maintained to the point where there are no working brakes to speak of.

She cited the U.S. Defense Advanced Research Projects Agency as a model, where projects can be started and stopped with a minimal amount of red tape.

PP2Cα was diluted in reaction buffer as appropriate to 10 µg/ml, and reactions were started by the addition of 4 mM pNPP (Sigma), incubated with varying concentrations of NPLC0393 for 2 h at room temperature, and stopped with a solution containing 1 N NaOH.

Antigen retrieval (10 m m-citrate buffer) was performed, followed by RNase treatment (20 μl RNase A (10 mg/ml), Thermo Fisher Scientific; 5 μl RNase T (10 units/ml), Thermo Fisher; 100 μl PBS (pH 7·4) and stopped with a 5 min treatment with NaCl solution (140 m m)).

A horseradish peroxidase conjugate bound to the detection antibody and colour development was accomplished by the addition of tetramethylbenzidine substrate and stopped with an acid stop solution.

After incubation with secondary antibody, plates were washed, incubated with 3,3′,5,5′-tetramethylbenzidine 3,3′,5,5′-tetramethylbenzidine equal volume of 1 mol l−1 H2substrate

Reactions were incubated for 5 min at 37°C and stopped with an equal volume of 20% glycerol, 1% SDS and 50 mM EDTA.

Reaction mixtures of 50 μl containing 15 μg of recombinant protein and 5 mM substrate were prepared in 20 mM HEPES (pH 7.5) and stopped with an equal volume of 0.8 M formic acid after 10 min of incubation at 25°C.

As he did, I watched a van draw up at speed and stop with a clever drifting skid on the gravel by the kiosk.

In the next three sessions parents and children worked on techniques like big and small pizzas and turning and stopping, with an eye toward gradually lessening the reliance on parents.