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Keeping your face clean and stimulated with a motorized skin care brush is one thing; aiming heat or beaming colored lights to clear acne is another.
DCs were generated from mouse bone marrow cells cultured with Fms-like tyrosine kinase-3 ligand and stimulated with a wide array of individual TLR agonists or simultaneously with pairs of combinations.
After 10 days of culture the myotube/cantilever constructs were placed in the detection system and stimulated with a 1 Hz pulse to elicit synchronous, detectable contractions.
After 10 13 days cantilever/myotube constructs were placed in the detection system and stimulated with a 1 Hz pulse train.
Figure 4A shows the raw data collected in free-run mode from myotubes cultured for 13 days and stimulated with a 5 volt DC pulse at a frequency of 1 Hz.
Splenocytes were harvested from 10 week old NOD females and stimulated with a defined concentration of anti-CD3/CD28 anti-CD3/CD28he presence or absence oforSN50 before detection of apoptosis with annexin-V staining (Fig. 4A).
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Freshly isolated splenocytes (1.5×106 cells) were added to each well and stimulated with an HA1 or HA2 subunit peptide pool at a concentration of 10 µg/ml.
The number of mIFN-γ secreting splenocytes from age-matched mice, as well as splenocytes from mice vaccinated with VLPs and stimulated with an irrelevant peptide or unstimulated was negligible (10 12 spots) following in vitro re-stimulation (data not shown).
To evoke excitatory junctional potentials (EJPs) in the muscles, the nerve was cut, placed in a suction electrode made of a glass capillary pulled in multiple steps and fire-polished, and stimulated with an A365 stimulus isolator (WPI, Sarasota, FL, USA).
To assess whether Shh-induced chemotaxis is mediated by Gi proteins, we pretreated monocytes with pertussis toxin (PTX), a specific inhibitor of Gαi/o, for 15 min and stimulated with an optimized concentration of Shh (1 μg/ml).
For T-cell IFN- γ release assays, irradiated DCs (1.6 × 10/200 μl per well) purified from nude mouse spleens using CD11c MACS beads (Miltenyi Biotec) were cocultured in 96-well plates with splenic T cells (1.6 × 10/200 μl per well) purified from BALB/c mice using CD90.2 MACS beads (Miltenyi Biotec), and stimulated with an anti-CD3 antibody (1 μg per ml) (BD Biosciences Pharmingen).
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