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We observed that Wnt3a promoted the ALP activity and staining in the culture medium.
Thus Neurod1, like Cux2, may be active in the germinative matrix of the DG, and staining in the gcl at postnatal stages reflects the activity of neurogenic factors during DG formation.
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These vascular-like structures evolved from a cord-like configuration to a more complex branched morphology, had a lumen and stained in the perivascular region for type IV collagen.
Sections were washed, permeabilized, and stained in the following manner.
Cardiospheres were fixed in ethanol/acetone and stained in the whole mount.
GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
GeneChips were washed and stained in the Affymetrix Inc. Fluidics Station 450 (Affymetrix, Santa Clara, USA) and hybridized.
The arrays were washed and stained in the Affymetrix Fluidics Station 450 and scanned using the Affymetrix GeneChip Scanner 3000 7G.
For immunohistochemistry, explants were cultured directly on fibronectin on 2-well chamber slides, fixed with 4% paraformaldehyde and stained in the whole mount.
Tissues were processed and stained in the histopathology laboratory in Nottingham University Hospitals Trust, UK.
GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix hybridization, wash, and staining Kit.
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