Sentence examples for and spotted on from inspiring English sources

Cultures grown overnight were diluted to an OD600 of 0.25, fivefold serially diluted and spotted on plates with or without relevant drugs.

For each gene, two cDNA probes (450 550 bp) were designed from different regions (coding region and 3′-untranslated region [3′-UTR], respectively) on the basis of simple criteria concerning length and sequence specificity and spotted on the macroarray.

Following a bioinformatic analysis of EST data using a semiautomated pipeline, 1885 representative ESTs (rESTs) were selected, to which oligonucleotides (three per EST) were designed and spotted on to a microarray.

Fermentation samples at different time were collected and spotted on a TLC plate (Merck, Germany) with 1-butanol/formic acid/water (4 6 1 v:v v) as a mobile solvent.

Independent dilution series were generated and spotted on a duplicate slide.

The reactions were cleaned with resin and spotted on SpectroChip arrays according to the manufacturer's recommendations.

Briefly, MVs were isolated and spotted on formvar-carbon coated grid for electron microscopy analysis.

Bacterial pellet were fixed in 2% PFA for 20 min at RT and spotted on POLYSINE™ slides (Menzel-Glaser).

Cells equivalent to an A660 nm×volume (ml) of 0.25 were harvested by centrifugation and spotted on a piece of filter paper (Schleicher & Schuell, Nr. 2294).

In addition 6 emm genes were cloned from strains with different M types and the corresponding proteins were purified and spotted on the chip.

Cultures were 5-fold serially diluted and spotted on drug-free and HU (2 mM)- and MMS (0.01% -containing YES plates using a purpose-built, replica-pin apparatus.