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These PCR assays have high sensitivity and specificity for the amplification of E. ruminantium DNA (6, 8 ).
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A dual signal amplification immunosensing strategy that offers high sensitivity and specificity for the detection of low-abundance biomarkers was designed on a 3D origami electrochemical device.
Fig. 6 Specificity of LAMP for the amplification of Zika virus.
The incorporation of locked nucleic acids (LNAs) into oligonucleotide primers has been shown to increase template binding strength and specificity for DNA amplification.
The amplification specificity for the genes of 18S, rbcL, GAPDH and ACTIN were determined by analyzing the dissociation curves of PCR products.
Two pairs of primers for each ZP gene were designed and tested for the amplification efficacy and specificity by electrophoretic analysis of the PCR products.
Melting curves of the amplified products were used to control for specificity of the amplification reaction.
To control for specificity of the amplification products, a melting curve analysis was performed.
As the amplification of DNA products are controlled by temperature, low-stringency cycles create sites for primer annealing, while high-stringency cycles augment the specificity of the nested amplification products [62].
Also, fusion curves obtained for each qPCR consistently showed a single peak, attesting for the specificity of the amplification (not shown).
To control for the specificity of the amplification products, a melting curve analysis was performed.
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