More than just digital replicas, these maps are linked to millions of bits of information, or attributes — the size of the pipes, the dates they were built and repaired, what they were made of — that can be called up and sorted with the click of a mouse.
After 48 hours, the cells were collected by trypsinization and sorted with the BD FACSVantage™ SE System at λex488 and λem522.
Sort by Duplicates Your cards will be grouped and sorted with the most number of duplicates at the top.
Embryos were collected from established lines and YFP expression confirmed by sorting with the COPAS Select.
In the case of sorting with the MoFlo cell sorter, prior to cell sorting and in keeping with the biosafety guidelines, CD4+ T cells were fixed with 1% PFA (experiments shown in Fig. 2A).
"It is a contractual issue with Alex and he needs to try and sort that with the club and come to an arrangement.
Work later in the year included the finishing of the fourth sail, balancing the fantail and sorting out teething troubles with the fantail gearing.
The lack of Cryptophyta in the sorted samples is explained by the fact that Cryptophyta, that display both red chlorophyll and orange phycoerythrin fluorescences, were not sorted with the settings used during the cruise.
BMCs were harvested 15 h later and LT-HSCs (Lin−cKit+Sca1+CD135−CD150+), ST-HSCs (Lin−cKit+Sca1+CD135−CD150−), MPPs (Lin−cKit+Sca1+CD135+CD150−) and myeloid progenitors (MPs) (Lin−cKit+Sca1−) were sorted with the ARIA III cell sorter (BD Biosciences).
ABM participated in the design of the coculture study, assisted with the coculture initial setup, assisted with counting and sorting the cells, assisted with immunofluoresence and flow cytometry procedures, analyzed and interpreted data and drafted and revised the paper.
