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For cell sorting, cells were treated as described above and sorted with a BDAria II FACS sorter (BD Biosystems).
Cells were analyzed and sorted with a FACSCanto or FACSAria (BD Biosciences).
Fed mosquitoes were transported to a laboratory and sorted with a mechanical aspirator, and each cohort was placed in a separate 0.2-L cage.
The cells were then suspended in PBS supplemented with 2% FBS and sorted with a FACSAria (BD Biosciences) into two populations (CD4+ CD127-CD25+ and CD4+ CD4+7- CD4+-).
IL-17 secreting IL-17 secretingre deTecellsaccording to the instructions of the manufacturer (Miltenyi Biotec) and sorted were a flow cytometer (FACSAria; Bdetectedngen).
In order to eliminate contaminating non-tumoral cells, recovered cells were stained with FITC-conjugated epithelial cell adhesion molecule (EpCAM) (DakoCytomation, Glostrup, Denmark) and sorted with a FACS Aria (Becton Dickinson).
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Euphilomedes carcharodonta were collected at the University of Southern California's Wrigley Marine Lab on Catalina Island, California by free diving, collecting sediment with an aquarium net, and sorting with a dissecting microscope.
Data were coded and sorted with themes identified using a constant comparison method.
Data was acquired at 10,000 events per sample and cells sorted with a BD Aria I and later Aria II, both using Diva software.
In immunofluorescence assay, before and after treatment, vimentin was expressed both in WT A549 and A549 sorted with a positivity of 100%.
The deposited material is gray, massive, and poorly sorted, with a dominant component of silt- to clay-sized fine ash (see "Grain-size characteristics" section).
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