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Embed specimens in paraffin and slice with a slicing machine at the thickness of 20 μm for the observation of angiogenesis and osteogenesis.
When it cools, divide the roll into ropes approximately 1⁄2 inch (1.3 cm) thick, and slice with a knife into pieces maybe 1 1⁄8 inches (2.9 cm) long.
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The apical 0.5 cm of the inflorescence tips was removed with tweezers, immediately embedded into 5% agar (Difco), and sliced with a Liner Slicer PRO7 vibratome (Dosaka).
Ms. Lohman returned to chopping and slicing with a few favorite knives, using a 1950s box grater she bought at a Salvation Army store and mixing with her two hands.
Briefly, the brain was dissected in ice-cold artificial cerebrospinal fluid (ACSF), mounted on an agar block and sliced with a vibratome (Leica VT1200s; Germany) at 4°C.
Brains were then rapidly dissected and sliced with a vibrating microtome (200 µm; Leica).
The segments were embedded in epoxy resin and sliced with a microtome (0.4 µm thickness).
Brains were dissected from mice and sliced with a McIlwain Tissue Chopper (Vibratome 800, Ted Pella, Inc., Redding, CA, USA) set to 300-μm sections.
When sampling the axillary branches, the middle portion of the internode was similarly quartered and sliced, with a sufficient number of the slices taken to approximate the tissue amount of the mature-culm samples.
Single berries at the three developmental stages around the onset of ripening (green hard, green soft and pale red) were collected at 9 wpf in 2011 and sliced with a microtome to be used for ROS detection.
Cut into 5 mm (1/4-inch) strips either while it is flat or by rolling up each sheet of dough and slicing with a sharp knife.
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