Self-assembled Ge nano-dots were produced by excimer laser annealing, and the statistical distribution of dot density and size was collected for different laser energy.
Peak height, area and sizes were collected by GeneMapper® software v3.7 and converted into FPC readable format after editing by the GenoProfiler® v2.1 (http://wheat.pw.usda.gov/PhysicalMapping/; [ 41]).
Air samples for toxin and particulate size were collected using two types of high-volume samplers (high-volume filter and high-volume air particle impactors equipped to capture aerosol particles by size) and a personal sampler of the subject breathing zone.
Because the data for physical activity, alcohol consumption, and body size were collected at baseline, we were also unable to address whether adherence to these guidelines later in life reduces mortality as it is possible that the women who complied with the guidelines have been engaging in healthy behaviors throughout their life.
A 5-mm spinal cord tissue containing the hemitransected region (medial), in addition to rostral and caudal regions of the same size, were collected and homogenized in 0.1 M phosphate buffered saline (pH 7.4) containing a protease inhibitor cocktail (PIC, Roche, Basel, Switzerland).
Each type of vegetation was selected according to size (large, medium, small), and four replicates of each size were collected; thus, 12 representative strains of each vegetation type and 36 total representative strains were selected.
Maps of 500 μm × 500 μm areas with 0.5 μm step size were collected and analysed for samples deformed in tension to 0%, 6 %, 10, 22.5% and 40% plastic strain.
Three samples each of M. tuberculata and L. varicus of comparable size were collected from each site for analysis.
For the determination of Rubisco, Chl and protein, leaf discs (0.61 cm2 in size) were collected at noon in full sun, frozen in liquid nitrogen, and stored at -80°C until assayed.
Leaf discs (0.63 cm2 in size) were collected at noon under full sun and immediately frozen in liquid N2.
