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Twenty-four single colonies were picked from YPD and 2-DOG plates for each strain, grown overnight in liquid YPD, and simultaneously prepared for flow cytometry or for long-term storage in 50% glycerol at −80°.
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The accessibility and openness of faculty make for exceptional community spirit, fostering scientific independence and simultaneously preparing young scientists for the necessary collaborative endeavors ahead.
So let's go big and create a comprehensive transportation plan now, one that is rendered elegantly and resourcefully to bring people together and simultaneously prepare for an uncertain energy future, something truly magical that is cherished by future generations as something their forefathers did presciently to ensure the resilience of their town for generations to come.
These functions robustly synchronize to diurnal variation oscillated by a circadian pacemaker, and simultaneously prepare for desynchronization from diurnal variation under stressful conditions.
During a scenario the trainer must pay full attention to the process and progress of the scenario, the behaviour of the trainees and simultaneously prepare the feedback that the trainees receive immediately after the scenario.
Sample and sample duplicate were simultaneously prepared and analyzed.
Triplicate non-selective media including TSA and R2A (Difco, France), were simultaneously prepared and inoculated by 0.1 ml aliquots of diluted sludge samples.
Erythrocyte RNAs (Fig 2A, lane 2 4) did not contain the two distinct ribosomal bands (28S and 18S) observed in simultaneously prepared peripheral blood mononuclear cells (PBMC) (Fig 2A, lane 5).
The round shape of the myofibrils indicates a proper cross section cut (as opposed to a section at an angle, which would produce an oval shape to the myofibril), and filament patterns of simultaneously prepared wild type samples were clearly in focus (Fig. 3A).
On days 7, 14, 21, and 28 cultures were simultaneously prepared for flow cytometry or for long-term storage in 50% glycerol at −80°.
To minimize the possible systematic experimental errors of the kinetic data, we simultaneously prepared and characterized all of the three mutants under the same experimental conditions, which allowed us to fairly compare their catalytic activity against -cocaine.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com