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Rats were sacrificed on day 1 post-injection and serial sections of the injected lateral ventricle were examined.
Expression patterns of the two Oscarella Wnt genes, determined using fixed pieces and serial sections of adult sponges, revealed striking complementary expression domains in the outer epithelial cell layer, the exopinacoderm (Figure 2).
Mice were sacrificed after 24 days post injection and serial sections of the liver and spleen cut at the distance of 150 µm from each other were hematoxylin-eosin (HE) stained.
They were embedded in paraffin and serial sections of 4 μm were cut for histological analysis.
Brains were mounted on a sliding-knife microtome and serial sections of 40 μm were placed free-floating in PBS.
Following dehydration in ethanol, they were embedded in paraffin by standard pathological procedures and serial sections of 5 μm were cut.
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After six weeks the number of lung metastases was determined by dissection and serial section of lung tissues from recipient mice.
After tissue processing and embedding, serial sections of 6 μm thickness were prepared and stained with hematoxylin and eosin.
To examine the MMP-9 expression and astrocytes' localization in brain cortex, immunofluorescence staining was performed on the first and second serial sections of the brain, which were deparaffinized, rehydrated, and washed with PBS.
Frozen tumor specimens were embedded in freezing medium and 15 serial sections of 40 mm were cut on cryostat and stored immediately in liquid nitrogen cooled vials for RNA isolation.
The number of intracardiac neurons in seven animals was assessed via counting of the nerve cells both on total hearts and in serial sections of the atrial walls.
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