Here we analysed NLL seed ESTs at the molecular level through the construction and sequencing of a cDNA library made from seed mRNA isolated at the major filling stage.
Sequencing of tryptic peptides by Edman degradation allowed the design of oligonucleotide primers for PCR, leading to the cloning and sequencing of a full length cDNA.
Here we report the identification and sequencing of a homologous PST gene cluster in L. wollei.
4) Cloning and sequencing of a huge number of copies of selected sequences is redundant, cost-ineffective, and time-consuming.
DNA barcoding involves the amplification and sequencing of a short universal molecular tag of approximately 650 bp from the 5' region of the mitochondrial cytochrome oxidase I (COI) gene [4] [5].
The tp53 genotype of DNA isolated from the tumor and control muscle tissue of the fish was determined by amplification and sequencing of a 200 bp fragment surrounding the M214K lesion.
The 5'UTR group of each of the HRV-C positive samples in the study was determined by amplification and sequencing of a region between 280 370 in the 5'UTR.
After bisulphite treatment and sequencing of a minimum of 5 clones for each sample, it was found that all tissue samples (normals and tumours) displayed almost complete methylation of the CpGs in this region (Figure 3).
An aliquot of DNA from all episodes of HAdV was designated for molecular typing based on nested PCR amplification and sequencing of a partial region of the HAdV hexon gene [31], [32]; 116 such samples were submitted to the Centers for Disease Control and Prevention laboratory performing these assays.
By cloning and sequencing of a HPV16+ CaSki cell small RNA library, we isolated 174 miRNAs (including the novel miR-193c) which could be grouped into 46 different miRNA species, with miR-21, miR-24, miR-27a, and miR-205 being most abundant.
Circumventing the construction and sequencing of a second linear library would be a significant cost advantage.
