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This SH3 minimotif analysis emphasizes the necessity of standardizing minimotif semantics and sequences in a well-modeled database with a query system that can be used to manage data from a collection of related studies.
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It introduces coding, but also problem solving, math, and sequencing in a way that many children with autism don't typically get to experience.
Indexed samples were pooled and sequenced in a single-flow cell on an Illumina HiSeq.
Six libraries were pooled together and sequenced in a single lane of a flow cell.
Four libraries are pooled together in equimolar ratios and sequenced in a single lane.
PCR products were cloned into pGEM-T easy vector and sequenced in a 3730 DNA analyzer.
All libraries, plus a control sample (no purification), were pooled and sequenced in a single lane of the HiSeq2500.
In the pre-genomic era, genes were identified and sequenced in a heuristic manner to address specific biological questions.
The recombinant expression vectors containing target genes were extracted as above, and sequenced in an ABI 377 automated sequencer.
Sequencing products were purified with isopropanol precipitation and sequenced in an ABI 310 Genetic Analyzer (Applied Biosystems).
BAC clone DNA was prepared and sequenced in an automated process using a 384-well format.
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