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There's movement around these: small limbs reaching, touching and separating in a semi-regular pattern, as though practising a butterfly or breaststroke.
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When the first magnetic layer had been attached to the bubbles, they were washed and separated in a magnetic field.
It lets me keep things organized and separated in a way that I've haven't found possible in other bags.
Tryptic peptides were desalted with Pierce C18 spin column (Thermo Fisher) and separated in a Proxeon EASY-nLC liquid chromatography system by applying a step-wise gradient of 0 85% acetonitrile (ACN) in 0.1% formic acid.
Alternatively, the sample can be prepared in an acidic buffer and separated in a basic buffer.
Equal volumes from each PCR reaction were loaded and separated in a 3% agarose gel.
20 μg of proteins were loaded and separated in a 10% SDS-PAGE and transferred to PVDF membrane.
Subsequently, lipids were extracted and separated in a neutral and phospholipid fraction by fractionation on a silica-G column as described [ 32].
Equal amounts of protein for each sample were loaded and separated in a 12% SDS-PAGE gel, and transferred to a nitrocellulose membrane.
For 2D analysis, 1D lanes were incubated for 15 min in 1% SDS and 1% β-mercaptoethanol and separated in a 16% Tricine/SDS gel (BN-SDS/PAGE).
Peak absorbance area at A202nm was used to quantify the different SGAs detected and separated in a frame of retention time between 5 and 15 minu.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com