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There's movement around these: small limbs reaching, touching and separating in a semi-regular pattern, as though practising a butterfly or breaststroke.
When the first magnetic layer had been attached to the bubbles, they were washed and separated in a magnetic field.
It lets me keep things organized and separated in a way that I've haven't found possible in other bags.
Tryptic peptides were desalted with Pierce C18 spin column (Thermo Fisher) and separated in a Proxeon EASY-nLC liquid chromatography system by applying a step-wise gradient of 0 85% acetonitrile (ACN) in 0.1% formic acid.
But because we have been able to be patient and separate in a respectful and considerate way.
Stable isotopes in biological samples are can be analyzed by gas isotope-ratio mass spectroscopy, in which samples are burned, converted into a gas, ionized, and separated in a magnetic field according to their mass.
Proteins collected from the apical surface of CNPEs were pooled and 5 ml of this sample was lyophilized overnight, suspended in 300 µl 1 X SDS-PAGE buffer without β-mercaptoethanol, and separated in a 15% SDS-PAGE gel.
Alternatively, the sample can be prepared in an acidic buffer and separated in a basic buffer.
Equal volumes from each PCR reaction were loaded and separated in a 3% agarose gel.
20 μg of proteins were loaded and separated in a 10% SDS-PAGE and transferred to PVDF membrane.
Subsequently, lipids were extracted and separated in a neutral and phospholipid fraction by fractionation on a silica-G column as described [ 32].
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