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Scrape out the fibre and seeds with a teaspoon and then cut each quarter in half yet again, also lengthways, giving 8 marrow "boats".
The best sources of explants were stems and seeds, with a maximum of callus induction of 75%.
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Seeds were examined at the indicated time points and seeds with an emerged, elongating radicle were counted.
The reconstructed landforms were simultaneously sprayed with a biodegradable emulsion to stabilise the surface matrix and seeded with a mixture of ~30 native trees, shrubs, and herbaceous species (~25,000 seeds per hectare).
The cell pellet was resuspended in 30 ml complete medium and seeded with a splitting ratio of 1 3.
CFSE-labeled NK cells were washed twice with PBS and seeded with a constant number of K562 cells (20,000) at different E T ratios (1 1, 3 1, 10 1. 30:10 1.
As presented in Table 1, Mesocarp of P. macrocarpa fruit showed the highest phenolic content (60.5 ± 0.18 mg GAE/g DW) followed by pericarp and seed with a value of 59.16 ± 0.037 and 47.70 ± 1.036 mg GAE/g DW respectively.
If there are any huge bare patches in the lawn, then the area should be rough raked and seeded with an all-purpose quality grass seed.
Cells from single mice were cultured individually, or pooled by the phenotype (WT, Aga2 mild, Aga2 severe) and seeded with an initial density of 1 × 10 cells per well in a 24-well plate.
Early African tribes would mix the coffee fruit and seeds with animal fat to make a sort of energy snack.
Explore winter fruits and seeds with photographer Anna Laurent, who will guide a group through the UC Botanical Garden to collect seed pods and photograph them in a studio environment.
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