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After 20 h incubation at 42°C the arrays were washed, dried and scanning using the Agilent scanner.
Hybridization using a GeneChip® Hybridization Oven 645 (Affymetrix) was followed by washing using the GeneChip® Fluidics Station 450 (Affymetrix) and scanning using the GeneChip®Scanner 3000 7G (Affymetrix).
The facility will perform the labeling, hybridization, and scanning using the HD2 Hx1 promoter tiling arrays (HD 2.1 M array) that span -8.2 kb to +3 kb around TSS of ~24,000 annotated genes.
Preparation of target products, hybridization and scanning using the GeneChip scanner 3000 were performed according to Affymetrix protocols using 5 μg of total RNA to prepare antisense biotinylated RNA.
Muscle expression pattern were assessed using 74 Porcine Genome Array which contains 24,123 probe-sets that interrogate 20,689 transcripts that were assigned to known genes [ 6]. Preparation of target products, hybridization and scanning using the GeneChip scanner 3000 were performed according to Affymetrix protocols using 5 μg of total RNA to prepare antisense biotinylated RNA.
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Strips were milled from these cores and scanned using the X-ray densitometer at Scion (Cown and Clement 1983).
The slides were exposed to phosphor screens for 1 h and scanned using the Cyclone Plus storage phosphor system.
About 50 μl of the suspended sample solution was pipetted out on to copper grip specimen mount, dried overnight, and scanned using the electron beams.
The arrays were washed, and scanned using the Affymetrix GeneChip® System.
Products were then fluorescently labeled and scanned using the Illumina Beadstation scanner.
Following hybridization, arrays were washed and scanned using the Affymetrix GeneChip System.
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