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Array hybridization and scanning, using a GeneChip 3000 scanner, were performed at the facility.
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Cores were stored frozen at −20 °C and scanned using a modified Seimens Somatom HiQ third-generation medical computed tomography (CT) scanner55.
The laser beam is focused on the substrate surface and scanned using a computer controlled galvanometer system.
The signal was developed using ECL Plus reagents (Amersham) and scanned using a Storm imager.
After hybridization, the microarray slides were washed and scanned using a Scan Array Express Lite (Perkin Elmer Life and Analytical Sciences).
The hybridized arrays were washed using the GeneChip Fluidics Station 450 and scanned using a GeneChip 3000 scanner.
Microarrays were exposed on a radioimager during 48 h and scanned using a Fuji BAS5000 machine (Fuji, France).
The gels were stained overnight with SyproRuby (Bio-Rad) and scanned using a Molecular Imager FX (Bio-Rad).
Following hybridization, arrays were washed and scanned using a GenePix 4000B Axon scanner (Axon Instruments, Union City, CA).
Samples were labeled using Cy-labeled random primers (9mers) and then hybridized onto the corresponding microarray platform and scanned using a GenePix 4000B scanner (Axon Instruments) as previously described[33].
The hybridizations were performed in fluor reversal for 24 hours in a hybridization chamber, washed, and scanned using a confocal laser scanner.
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