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Electrochemical impedance spectra (EIS) were measured from a CHI 660E electrochemical workstation (Chenhua Inc., Shanghai), applying a 10-mV AC signal and scanning in a frequency range between 1 MHz and 1000 Hz at different forward applied bias.
The AuNP samples were prepared by drop-coating the pelletized AuNPs on a glass slide and scanning in a 2θ region from 30° to 90° at 0.01° per minute with a time constant of 2 s using a D8 Advance (Bruker-axs) X-ray diffractometer.
Detection was performed using fluorescently labeled secondary antibodies at 0.1 μg/ml (LI-COR) and scanning in a LI-COR Odyssey system.
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Images were recorded in digital imaging plates and scanned in a Ditabis Micron scanner (Pforzheim, Germany).
The specimen was then placed in the diffractometer and scanned in a continuous mode from 7° to 70° with a scanning rate of 0.05° s−1.
Bone samples were obtained from the human proximal tibia, canine distal femur, rat tail, and pig spine and scanned in a micro-CT scanner.
The specimens were viewed using transmission electron microscopy [TEM] (JEM.1200ex, JEOL Ltd., Herts, England, UK), and images were recorded in digital imaging plates and scanned in a Ditabis Micron scanner (Ditabis AG, Pforzheim, Germany).
The Zn-TAP NPs were dissolved in distilled water (1 mg/mL) and scanned in a Perkin Elmer Lambda 25 UV Vis spectrometer at 25 °C in the range of 250 650 nm.
The gel was dried and exposed in a phosphor screen and scanned in a Phosphor Imager (Fuji Photo Film, Japan).
After staining, gels were washed for 1 hour in 10% methanol/7% acetic acid, and scanned in a Typhoon 9410 (GE Healthcare).
The slides were then air-dried and scanned in a microarray scanner (Genepix Personal 4100A, Axon Instruments, Union City, CA).
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