Cores were stored frozen at −20 °C and scanned using a modified Seimens Somatom HiQ third-generation medical computed tomography (CT) scanner55.
The tested samples were then sectioned into two halves and scanned using a scanner, optical and scanning electron microscopes to understand the failure patterns.
Microfil consisting of radio-opaque polymer was perfused through the mice and scanned using a Bench Top CT system for micro-computed tomography.
The laser beam is focused on the substrate surface and scanned using a computer controlled galvanometer system.
The signal was developed using ECL Plus reagents (Amersham) and scanned using a Storm imager.
The gels were stained overnight with SyproRuby (Bio-Rad) and scanned using a Molecular Imager FX (Bio-Rad).
Following hybridization, arrays were washed and scanned using a GenePix 4000B Axon scanner (Axon Instruments, Union City, CA).
The hybridized arrays were washed using the GeneChip Fluidics Station 450 and scanned using a GeneChip 3000 scanner.
The hybridizations were performed in fluor reversal for 24 hours in a hybridization chamber, washed, and scanned using a confocal laser scanner.
Fifteen micrograms of labeled aRNA were fragmented and hybridized to Affymetrix GeneChip Human Genome U133 Plus 2 arrays and scanned using a GeneChip Scanner (Affymetrix).
After hybridization, the microarray slides were washed and scanned using a Scan Array Express Lite (Perkin Elmer Life and Analytical Sciences).
