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At baseline examination, serum was extracted within 1 h from venipuncture and samples were thereafter stored at −80 °C [ 26].
Rice seedlings were stressed at 4 o C for 30 min, 2, 4, 8 and 24 h, and samples were thereafter hybridized to the Affymetrix GeneChip® Rice Genome Array.
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The average amplitude spectra (square root of power) across all the 4 seconds long samples were thereafter calculated and smoothed.
The blood samples were thereafter transported at room temperature to the BGA and to the central laboratory for analysis.
The samples were thereafter stepwise dehydrated in ethanol (0%, 25%, 50 %, 75 %, 96 99.6%) and then critical-point dried.
DNA samples were thereafter sent to the Unité des Rickettsies in Marseille, France.
All samples were thereafter coded using a study number as an identifier.
Five samples were thereafter removed from the dataset, applying a cutoff likelihood ratio of 1000 [ 22].
Samples were thereafter diluted 1 10 with denaturing loading solution (98% formamide, 9.8 mmol/L EDTA (pH 8.0), 0.098% bromphenol blue and xylene cyanol).
Cell medium from air-liquid interface cultures exposed to normoxia or hypoxia were incubated with neutrophil elastase, and the elastase activity in the samples was thereafter analysed with a chromogenic assay.
The poultice was then removed and the sample was thereafter rinsed with water and dried in the air.
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