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Within 4 h of collection, study personnel transported the samples to the central processing laboratory at the Shanghai Cancer Institute for processing, and samples were later shipped to the NCI repository on dry ice via Federal Express.
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TE redissolved DNA frozen in 96-well plates (~200 ng DNA in 50 μl/well) was shipped to Affymetrix, Inc., Santa Clara, CA (Yuker Wang), where Study 1 sample pairs were analyzed on a 20K MIP array platform and Study 2 samples were later analyzed on a 300K MIP array platform, and all pre-processed data returned for further determination of allele-specific copy number aberrations (CNA) [ 17– 20].
The frozen samples were later sectioned and probed for CD31 and TUNEL.
Sediment samples were later thawed and sifted to remove rocks and larger particulates.
Samples were later dried and homogenised using a mortar and pestle.
Following an international outcry, the samples were later returned, and the men put on trial in absentia – along with five Egyptian officials accused of helping them to illegally access the pyramid.
Samples were later transferred and stored in a −70°C freezer.
Samples were later thawed, and a serum pool was generated for each herd, derived from up to 30 individual sera (each 10 μL).
The samples were later identified in the laboratory and prepared for analysis.
The samples were later transferred to the analytical laboratory and stored at -20°C for tramadol determination.
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