Sentence examples for and revealed with a from inspiring English sources
Western blot membranes were successively incubated with anti-Hfq antibodies, secondary antibodies coupled to alkaline phosphatase (Sigma) and revealed with a NBT/BCIP Reagent Kit (Molecular Probes).
Western blots were carried out using horseradish peroxidase-conjugated goat anti-rabbit and anti-mouse antibodies and revealed with a chemiluminescence detection system by Pierce.
Macrophages were stained using a rat anti-mouse Mac-3 monoclonal Ab (BD Biosciences, dilution 1/75) and revealed with a secondary biotin-conjugated goat anti-rat Ab (Jackson Immunoresearch, dilution 1/200).
Following electrophoresis, proteins were transferred by electroblotting to a PVDF membrane (Bio-Rad) and revealed with a mouse immune serum to Ebola ZGP at a 1∶1500 dilution as the primary antibody followed by a horseradish peroxidase-conjugated goat anti-mouse secondary antibody diluted 1∶7500.
After antigen retrieval, FADD antibody (mouse monoclonal anti-hFADD (clone 64A6, Abcam, Cambridge, UK), 1 : 50 dilution) was applied and revealed with a streptavidin-biotin-peroxidase kit (BIOSPA, Abcys, Paris, France).
Actin was used as loading control and revealed with a goat anti-actin primary antibody (Santa Cruz Biotechnology, Dallas, TX) and rabbit anti-goat Alexa Fluor 680 secondary antibody (Invitrogen).
Membranes were blocked in 5% nonfat dry milk, incubated with primary antibodies, detected by the appropriate secondary antibodies, and revealed with an enhanced chemiluminescence system (Amersham Biosciences).
The membranes were washed again with TBST (10 min × 3) and revealed with an enhanced chemiluminescence system (ECL kit; Pierce Biotechnology).
For signal amplification, cells were then incubated with an anti-Cy3 antibody (Invitrogen) and revealed with an anti-mouse-TRITC antibody (Invitrogen).
For quantitative real-time RT PCR, amplification was performed in a LightCycler™ system (Roche, Mannheim, Germany) and revealed with an SYBR Green™ kit (Quantitect™ SYBR Green PCR, QIAGEN).
Cytosolic homogenates from hearts submitted to the different experimental protocols were immunoprecipitated with anti-VDAC antibody, resolved by SDS/PAGE and revealed with an anti-P-GSK-3 β or anti-GSK-3 β antibody.
