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However, endocardial cells were present and retained expression of general vascular endothelial markers.
Immunohistochemical staining showed the tumor cells were Thy1 positive and retained expression of human Bcl-2 (Figure 8).
These cell lines were derived from parental MCF7 cells upon prolonged treatment with tamoxifen and retained expression of ERα.
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The resultant cell lines express an HCEC-specific transcriptional fingerprint, and retain expression of the corneal endothelial temperature-sensitive potassium channel, suggesting that significant dedifferentiation does not occur as a result of these modes of immortalization.
Macrophages and fibrocytes cultured for 7 days, had low or undetectable expression of CD33, CD35, and CD93, but retained expression of S100A8/A9 (Figures 1 and 4).
Spheroids cultured in undifferentiated spheroids of 3 and 10 days retained expression of CD44, without expression of differentiation markers.
Only one of the 20 asbestos-induced MMs showed loss of p53 expression (Fig. 3B), and that single sample retained expression of Arf, consistent with our previous work showing a reciprocal pattern of inactivation of Tp53 in asbestos-induced MMs from Nf2 mice that had retained expression of Arf [5], [6].
Cells derived from the finch blastoderm at oviposition and cultured in the presence of a MEK inhibitor and Leukemia inhibitory factor (LIF) retained expression of the pluripotent markers; Nanog, PouV expression, and alkaline phosphatase (AP) activity.
Cell karyotyping analysis confirmed these cells were of murine origin and immunohistochemical analysis established they retained expression of antigens consistent with the original tumors.
Western blot analysis showed that transformed IAR-6-1 epithelial cells retained expression of epithelial E-cadherin and did not express mesenchymal N-cadherin (Figure S1).
Considering the retained expression of Oct4 and Nanog, we were interested to see the effect on gene transcription of these co-occupied genes in absence of Ring1b.
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