Detailed SGP dataset annotation statistics is available at SGP data resource > Data and results > Annotations > SGP full annotation, statistics.
The annotation of the clustered ESTs using GO terms is accessible through links at the Salmon Genome Project website [ 11] in the annotation section (Data and Results > Annotations > SGP Intestine-Gills).
Results: Annotations and gene predictions were successfully added to more than 10,000 contigs for potential use in downstream primer design.
The raw datasets supporting the results of this article, including unfiltered assembly results, protein predictions, BLAST results, annotations, and orthology files are available in the GigaScience repository [ 13].
To visualize Blast [ 62] results, annotations and to search for sequences by gene keywords or GO terms, the ESTtik database records can be accessed using 7 query pages combining PerlCGI, HTML, Javascript and Flash technologies.
All sequences, blat and blast results, annotation tables were loaded with custom python scripts into a MySQL database.
The up regulated gene lists obtained at a SAM delta of 2.0 (Table 1) were uploaded to the tool and the resulting annotations examined.
For optimal results, annotation and imaging was performed within 1 month of staining.
Files are also provided to download enrichment results, annotation tables, and PO diagrams in SVG format.
Results for every unigene, including constituent reads, expression data, BLAST results, annotation evidence and relevant links are summarized on individual pages available through MAGPIE.
These updates are integrated into the genome annotation processes at NCBI and Ensembl, resulting annotation is compared and the CCDS analysis process confirms the update and increments the version number of the CCDS ID.
