RAG2−/− mice were irradiated with 20 gy and reconstituted with 5×106 cells from a 1∶1 mixture of either WT∶B6.SJL or p110δD910A∶B6.SJL bone marrow.
Stock solutions of the amino acid mixture were desiccated under gaseous nitrogen and reconstituted with sterile saline at a concentration of 100µCi/µl.
Tryptic peptides were completely dried in a SpeedVac and reconstituted with 10 ml of 0.1% TFA.
Pravastatin sodium salt (Sigma, UK) was obtained in a lyophilized form and reconstituted with sterile water.
Hooded rats bearing a syngeneic leukaemia were irradiated and reconstituted with allogeneic bone marrow.
Fractions were collected in a 70 min run, dried, and reconstituted with nanopure water.
The organic layer was removed and reconstituted with the mobile phase.
Wildtype and Cd34−/− animals were irradiated and reconstituted with bone marrow from wildtype Ly5.1 congenic animals.
The solvent was evaporated and reconstituted with water.
Plasma samples are preextracted with methanol, dried with speed vacuum, and reconstituted with 0.1% trifluoroacetic acid.
The pellet was washed with 70% ethanol twice, dried and reconstituted with RNase free water.
