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The protein bands were visualized by Coomassie Brilliant Blue G250 staining and quantitated using the NIH ImageJ program (http://rsb.info.nih.gov/ij/).
Protein bands were detected by chemiluminescence (ECL, Amersham Pharmacia) and quantitated using the Image J software.
The fOSs isolated was labeled with 2-amino pyridine, and quantitated using the HPLC as described before [48], [49].
DNA was checked on a 1% agarose gel and quantitated using the PicoGreen method (Invitrogen, Carlsbad, CA).
Membranes were scanned at 700 nm or 800 nm using the Odyssey Infrared Imaging System (LI-COR Biosciences), and quantitated using the accompanying software package (Odyssey V 3.0).
PCR of bisulfite-treated DNA results in pools of products containing both methylated and unmethylated DNA that can be discriminated and quantitated using the pyrosequencing method.
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S11494; Molecular Probes Inc., now part of Invitrogen Corporation) to minimise interassay variability and were quantitated using the FluorImager/Typhoon and ImageQuant software (Molecular Dynamics, now part of GE Healthcare, Little Chalfont, Buckinghamshire, UK), as previously described [ 16, 24, 27].
The image was then analyzed and signals quantitated using the ImageQuant 5.0 software package.
Supernatant was collected and protein quantitated using the BCA protein assay kit (#23227, Pierce).
LPS was extracted from H. pylori strains (26695) using the phenol-water extraction procedure previously described [ 27], and was quantitated using the purpald assay [ 28].
Briefly, proteins were extracted from the cultured cells and then quantitated using the bicinchoninic acid (BCA) assay kit (Pierce, Rockford, IL, USA), with BSA as a standard.
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