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Using 32P-postlabeling assays, IQ-DNA adducts were isolated and quantitated in the liver, small intestine, and large intestine.
Whole IgG molecules were detected and quantitated in the COS-7 cell supernatants using a direct ELISA, as described in previous papers [ 29, 35, 37].
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The luciferase activity of the cultures was measured and quantitated in a luminometer using the Dual-Luciferase Reporter after System (Promega) after 48 h transfection.
The 3 bromophenols were detected in all samples, and 7 of the 15 HO-PBDEs were identified and quantitated in all or some of the samples.
As shown in Figure 3, derivations of the endocannabinoids were detected and quantitated in mES cells and EBs at days 7 and 14.
The results of such an experiment are shown in the form of a colony formation assay in Figure 1C and quantitated in Figure 1D.
At the end of the incubation time, the cells that invaded into the lower chamber were detached, lysed, stained with CyQuant and quantitated in a fluorescent plate reader.
Samples were prepared from biological triplicates and quantitated in duplicate.
DNA was extracted (Hi-speed Maxi-prep Kit, Qiagen) and quantitated in triplicate (Nanodrop).
Reaction was imaged and quantitated in a LI-COR Odyssey image analyzer.
The mRNA, cRNA and vRNA levels were measured from 0 to 24 hours (Figure 2A) and quantitated in Figure 2B.
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