The phrase "and quantitated by using" is not correct in standard written English; the correct term is "quantified." You can use it in contexts where you are describing a process of measuring or assessing something quantitatively.
Example: "The samples were analyzed for their chemical composition and quantified by using spectrophotometry."
Alternatives: "and measured using" or "and assessed by means of".
Blots were visualized and quantitated by using a Li-Cor Odyssey Infrared Imager (Li-COR Biosciences, Lincoln, NE). 25 nM of siRNA specific for MDC1 or a control siRNA was transfected with Lipofectamine 2000 (Invitrogen, Carlsbad, CA), according to supplier's instructions.
Wound areas at each time point were analyzed and quantitated by using ImageJ software.
The amplified products were gel electrophoresed and quantitated by using Quantity One software by Bio Rad gel documentation system.
The migration ability was recorded at 24 h using a phase contrast microscope at 10 × magnification and quantitated by using the Wimscratch software (Wimasis Image Analysis, Germany).
The finalcDNA product was used for subsequent cDNA amplification bypolymerase chain reaction.cDNA was amplified and quantitated by using SYBR Green PCR reagentsfrom PE Applied Biosystems according to the manufacturer'sinstructions.
The apoptotic cells were detected and quantitated by using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) system (Promega, Madison, WI, USA) and propidium iodide (PI /annexin V method (R&D Systems, Minneapolis, MN, USA) according to the manufacturer's instructions.
The RNA was quantitated by using a RiboGreen RNA quantitation kit (Molecular Probes Inc., now part of Invitrogen Corporation, Carlsbad, CA, USA), dissolved in diethylpyrocarbonate-treated H2O, and stored at -80°C until use.
The expression levels of BAFF, BAFF-R, TACI, NF-IL6, NF-IL6β, NF-κB1, NF-κB2 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were quantitated by using a method described previously [ 6].
Cells were quantitated by using image analysis and the quantity of blood vessels surrounded by collagen XVIII-expressing basement membrane was evaluated on a 4-tiered scale from the captured images of the invasive front and the tumour stroma (Väyrynen et al, 2012, 2013).
Oestrogen receptor- α protein levels were quantitated by using a 0 5 scale (O=lowest and 5=highest) to determine the percentile of cells showing nuclear staining, and a 0 3 scale to determine the staining intensity.
