After cloning, each monomer was obtained by restriction digestion with Eam 1104I and isolated and purified with a DNA gel extraction kit.
The PCR products were confirmed by electrophoresis in 0.8% agarose gel and purified with a Gel DNA Mini Purification Kit (Tiangen, Beijing, China).
A highly active truncated soluble protein was expressed and purified with a nickel HiTrap chelating metal affinity column to apparent homogeneity based on Coomassie blue-stained SDS PAGE.
The α-subunit of PC from Synechocystis sp. PCC6803 was produced in an N-terminally His-tagged form according to [3] and purified with a modified protocol from [5].
Plasmids containing the PCR product were isolated using an alkaline lysis protocol (Birnboim and Doly [1979]), screened using PCR with primer pairs: (AG 10 or (AC 10/SP6 or T7), and purified with a PureYieldTM Plasmid Miniprep System (Promega).
Total RNA was extracted from leaves of transgenic and wild-type rice plants using TRI REAGENT® Molecular Research Centerr, www.mrcgene.com) and purified with a Qiagen RNeasy Mini Kit (Qiagen, www.qiagen.com).
In this study, recombinant Oryza sativa L. cv Tainung 67 human serum albumin (OsrHSA) was expressed and purified with a single-step polyethylene glycol (PEG /phosphate aqueous biphasic system (ABS).
The microwave-assisted extraction of Undaria pinnatifida and Sargassum fusiforme were separated and purified with a non-aqueous two-phase solvent system composed of n-hexane acetonitrile methanol (5:5:3, v/v/v).
Before labeling, DNA was digested with DpnII and purified with a DNA Clean-up and Concentrator 5 Kit (Zymo Research).
The backbone vector pCTCON-T was digested with Xcm I and purified with a Tiangen gel purification kit to reduce self-ligation.
The product was run on a 1.0 % agarose gel and then removed from the gel and purified with a Jetsorb DNA extraction kit.
