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Then, the cells were harvested on filter paper and processed for the determination of of H-Tdr incorporation by liquid scintillation.
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Some of the tissues were lysed for protein isolation and then processed for the determination of signaling pathway proteins using Western blot method.
Following sacrifice, the cecal materials, livers and spleens were collected and processed for determination of Salmonella titre as described below.
The insoluble portion of the curcuminoids was pelleted and the supernatant removed and processed for concentration determination as described.
At each sampling, three and five replicates of 5 individuals were collected and processed for total lipid determination and mRNA gene transcription measurement, respectively, and 10 animals were processed for Nile red determination.
Drug analysis in biological fluids is a very important process for the determination of the physiological performance of a drug for toxicological and clinical purposes [ 1].
For determination of DNA synthesis, cells were incubated with H-thymidine (1 μCi ml 1) for 1 h, washed twice with phosphate-buffered saline and processed for scintillation counting.
A portion of the liver was immediately fixed in formalin for histological analyses, and another portion was processed for determination of oxidative-stress-related products (see below).
The remaining half of each kidney was processed for determination of Hyp and Pro levels.
Samples of the ventrolateral medulla were processed for determination of ATP concentration as described previously [17], using an ATP bioluminescence assay (Roche Diagnostics GmbH, Mannheim, Germany).
In their study, the fuzzy analytic hierarchy process was utilized for the determination of significance coefficients of attributes.
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