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Turn left at the visitors' center and proceed for three and a half miles on Rockwell Road to the Jones Nose trailhead and parking area.
This sample is first diluted with 50 μl PBS and then mixed with equal volume of 1% LMA and proceed for the experiment (Bajpayee et al. 2005).
The highways continue east for 11 mile to Cestos, another unincorporated place, and proceed for 8 mile to a junction with US-183.
Routinely, 24 h urine specimens are collected in brown bottles with 6 N HCl as preservative and proceed for analyses without delay.
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They passed a jumble of signs warning them in English about quicksands, rising tides and hidden channels, crossed the salt marshes strewn with empty cockle-bags, and proceeded for over two miles across the mush exposed by the low tide.
The products were then dried in a vacuum oven at 100 °C for 3 h and proceeded for characterization.
We attempted the cloning of full-length coding sequences (CDSs) using conserved primers 5UTR-F/3UTR-R (Table 1) and proceeded for sequencing.
The reaction was started by switching on a 30 W fluorescent lamp placed 30 cm above test-tubes and proceeded for 15 min.
The cells were then paraformaldehyde-fixed and proceeded for immunofluorescence as described above.
Intestines were rolled in the "Swiss rolls" configuration and proceeded for paraffin embedding [32].
Thirty minutes after first tetanization slices were fixed in 4% PFA and proceeded for cryosectioning, immunostaining and confocal imaging.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com