The phrase "and probing in a" is not complete and lacks context, making it difficult to assess its correctness in written English.
It could be used in contexts where you are discussing an action or process that involves investigation or inquiry, but it needs to be part of a complete sentence.
Example: "The researchers were focused on understanding the phenomenon and probing in a systematic manner."
Alternatives: "and investigating in a" or "and exploring in a".
We studied walking in a four-arm olfactometer and probing in an agar substrate in response to olfactory stimulation by fruit odours.
Reactions contained 5 µl of RNA template, 1× TaqMan one-step RT-PCR master mix (Applied Biosystems, USA), 1× MultiScribe and RNase inhibitor mix (Applied Biosystems) and 0.5 µM of each primer and probe in a total reaction volume of 25 µl.
The assay provides for the direct detection of a signal related to the base specific triplex complex formed by the duplex target sequence and probe in a homogeneous solution.
Affymetrix gene chips harbour several oligonucleotide probe types – prevalent unique probe sets (_at) accompanied by identical probe sets (_s set) and probes in a mixed probe set (_x set).
The final concentrations of primers and probes in a total of 50 μl of reaction mixture were 0.3 and 0.1 μ M, respectively.
The cDNA was mixed with the TaqMan® Assays-on-Demand™ primers and probe in a 2× TaqMan Universal Master Mix (ABI) and run in 10 μl triplicate PCR in a 384-well tray (ABI).
The detection of viruses was done by Real Time PCR, using the Taqman System® (Applied Biosystems, New Jersey, USA), with specific primers and probes in a thermal cycler (7300 Real Time PCR system® - Applied Biosystems).
For qRT PCR, 2 μl of the cDNA was amplified using TaqMan Universal Master Mix with optimised concentrations of CAR primer sets and probes in a standard optical 96-well reaction plate.
Because the amplified fragment length in the case of whole-genome hybridization was 100-250 bp and probes in a probe set were concentrated in a ~300-500 bp region near the 3'-end of genes, false positives by amplification polymorphisms should be clustered.
The IS6110_T primer pair generated a PCR product from M. bovis, M. wolinskyi and M. goodii using conventional PCR; however, using the primers and probe in a real-time PCR assay resulted in a positive reaction to only M. bovis and M. wolinskyi (Table 1).
