Sentence examples for and probes in a from inspiring English sources
The final concentrations of primers and probes in a total of 50 μl of reaction mixture were 0.3 and 0.1 μ M, respectively.
For qRT PCR, 2 μl of the cDNA was amplified using TaqMan Universal Master Mix with optimised concentrations of CAR primer sets and probes in a standard optical 96-well reaction plate.
The detection of viruses was done by Real Time PCR, using the Taqman System® (Applied Biosystems, New Jersey, USA), with specific primers and probes in a thermal cycler (7300 Real Time PCR system® - Applied Biosystems).
Affymetrix gene chips harbour several oligonucleotide probe types – prevalent unique probe sets (_at) accompanied by identical probe sets (_s set) and probes in a mixed probe set (_x set).
Because the amplified fragment length in the case of whole-genome hybridization was 100-250 bp and probes in a probe set were concentrated in a ~300-500 bp region near the 3'-end of genes, false positives by amplification polymorphisms should be clustered.
Ovarian samples included 7 normal ovarian tissues and 41 ovarian tumors ranging from Stages I to IV. MPL gene expression was measured using FAM-TAMRA-labeled primers and probes in a 7900HT thermal cycler using a standard 40-cycle profile with 9600 emulation.
Reactions contained 5 µl of RNA template, 1× TaqMan one-step RT-PCR master mix (Applied Biosystems, USA), 1× MultiScribe and RNase inhibitor mix (Applied Biosystems) and 0.5 µM of each primer and probe in a total reaction volume of 25 µl.
The assay provides for the direct detection of a signal related to the base specific triplex complex formed by the duplex target sequence and probe in a homogeneous solution.
Using a planar microfluidic device, our group reported on a single microchannel Western technique that unifies protein separation and probing in a single microfluidic channel.
The cDNA was mixed with the TaqMan® Assays-on-Demand™ primers and probe in a 2× TaqMan Universal Master Mix (ABI) and run in 10 μl triplicate PCR in a 384-well tray (ABI).
We studied walking in a four-arm olfactometer and probing in an agar substrate in response to olfactory stimulation by fruit odours.
