Sentence examples for and probe in a from inspiring English sources

The assay provides for the direct detection of a signal related to the base specific triplex complex formed by the duplex target sequence and probe in a homogeneous solution.

Reactions contained 5 µl of RNA template, 1× TaqMan one-step RT-PCR master mix (Applied Biosystems, USA), 1× MultiScribe and RNase inhibitor mix (Applied Biosystems) and 0.5 µM of each primer and probe in a total reaction volume of 25 µl.

The cDNA was mixed with the TaqMan® Assays-on-Demand™ primers and probe in a 2× TaqMan Universal Master Mix (ABI) and run in 10 μl triplicate PCR in a 384-well tray (ABI).

The IS6110_T primer pair generated a PCR product from M. bovis, M. wolinskyi and M. goodii using conventional PCR; however, using the primers and probe in a real-time PCR assay resulted in a positive reaction to only M. bovis and M. wolinskyi (Table 1).

Affymetrix gene chips harbour several oligonucleotide probe types – prevalent unique probe sets (_at) accompanied by identical probe sets (_s set) and probes in a mixed probe set (_x set).

Using a planar microfluidic device, our group reported on a single microchannel Western technique that unifies protein separation and probing in a single microfluidic channel.

The final concentrations of primers and probes in a total of 50  μl of reaction mixture were 0.3 and 0.1  μ M, respectively.

The detection of viruses was done by Real Time PCR, using the Taqman System® (Applied Biosystems, New Jersey, USA), with specific primers and probes in a thermal cycler (7300 Real Time PCR system® - Applied Biosystems).

For qRT PCR, 2  μl of the cDNA was amplified using TaqMan Universal Master Mix with optimised concentrations of CAR primer sets and probes in a standard optical 96-well reaction plate.

Because the amplified fragment length in the case of whole-genome hybridization was 100-250 bp and probes in a probe set were concentrated in a ~300-500 bp region near the 3'-end of genes, false positives by amplification polymorphisms should be clustered.

The sequences of the primers and probe in the (A) DENV1, (B) DENV2, (C) DENV3, and (D) DENV4 RT-PCR plotted against their respective non-targeted serotypes.