Hanging drop cultures are the widely used culture approach for both embryonic (including embryonic stem cells) and adult tissues, and have been previously successfully applied to culture intact fetal mouse testes and adult murine seminiferous tubules (Szczepny et al, 2009).
Students could include both peers of the respondent and students who previously successfully negotiated the particular year of study.
Macular oedema presenting de novo immediately following cataract surgery can spontaneously resolve with a good result, and patients with previously successfully treated macular oedema do well with cataract surgery [ 22].
To this end we, and others, have previously successfully applied fast low angle shot (FLASH) MRI imaging (Cho et al. 2010; Forstmann et al. 2010, 2012; Keuken et al. 2013; Massey et al. 2012).
The Delphi technique [ 27] has been used to establish a consensus between acupuncturists regarding the standardized diagnostic criteria and treatment guidelines, as previously successfully used for acupuncture treatment of lateral epicondylitis [ 28].
To dissect the contributions of each of PARP-1's domains to mADPR accumulation under conditions of O/N stress in vivo, we generated a series of human PARP-1 mutants that had been previously successfully expressed and characterized in vitro (Figure 2 and legend).
The psychiatric diagnosis was made according to DSM-IV TR [ 11]. by clinicians using ANTAS, which is a semi-structured interview [ 12, 13] previously successfully used and validated in this population.
Using protein engineering, we previously successfully reduced xylitol accumulation and improved ethanol production by reversing the dependency of XDH from NAD+ to NADP+.
Lorig has previously successfully introduced the generic and group-based approach in programme design found in this study [ 12, 38].
This approach was previously successfully used by us and others to provide orthogonal identification of histone PTMs [ 15, 28, 29].
