Anti-V3 mAbs exhibited the most breadth and potency in neutralizing the evolving viruses.
In order to understand the mechanisms behind this sensitisation process we have used anisomycin, an agonist of the JNK pathway in mammalian cells, which is often used in studies involving JNK because of its specificity and potency in activating the JNK pathway.
Analysis of these four scFv-PE38 immunotoxins indicated a correlation between antigen binding affinity and immunotoxin potency in the cell killing assay with the exception of the scFvPE38 immunotoxin derived from the antibody BC3.
Further optimization using structure-based design led to inhibitors with pM affinity for FXIa, excellent selectivity against a panel of relevant serine proteases, and good potency in the activated partial thromboplastin time (aPTT) clotting assay.
However, there is no correlation between the scores obtained in these tests and the potency in the RD50 bioassay.
Here, we investigate the impact on antigenic locations of estimating virus avidity and serum potency in the BMDS model.
In humans however, while showing much promise in preclinical models, DNA vaccines have shown reduced and disappointing potency in the clinic (reviewed in Ferraro et al, 2011).
There is a significant correlation between the clinical efficacy of antidepressant drugs and their potency in the FST, this was not found in any other model [ 34, 36].
The amide was replaced with a methylene linkage in order to lower desolvation, and the potency in the cellular assays was also found to improve by replacing the pyrrolidine with a tert-butyl group (i.e., 36).
Norephedrine-based compound 3 and norpseudoephedrine-based 4 had improved maximal activity over 1 and slightly improved potency in the C3H10T1/2 assay, as did the prolinol derivative 5.
