Sentence examples for and plated in the from inspiring English sources

For a comparison of same-species haematopoietic progenitor expansion, MNCs were extracted from rat whole blood via Ficoll gradient separation and plated in the same manner (separate platings of 10 and 10 cells).

Briefly, HEK 293 cells were suspended in DMEM and plated in the top chamber.

Cells were washed and plated in the presence of each indicated condition at a concentration of 50,000 cells/well (1 ml total) in a 24-well plate.

2.5×105 peripheral blood mononuclear cells (PBMC) were separated, washed twice and plated in the T-SPOT.TB plates stimulated with or without RD1 selected peptides and PHA, as described above and previously [14], [15].

Peritoneal exudate cells (PECs) were harvested from the healthy, inbred strain of Balb/c mice (8 10 weeks old, 20 22gms) using chilled serum-free RPMI 1640 medium and plated in the wells of 24 well plate (Nunc, Denmark).

When the DX-producing strain is both cultured and plated in the absence of inducer the resulting CFUs appear indistinguishable from CFUs produced in the control stains, and only minor differences are observed when the DX-producing strain is cultured in liquid media without inducer but plated onto solid media containing the arabinose inducer.

The method of lengthening over a rod has been described [ 2, 21], and more recently lengthening and plating in the tibia as well [ 22].

Mononuclear cells were resuspended in 1 2 ml AIM-V® medium (Gibco, Invitrogen) counted, viability assessed and plated in duplicate in the ELISPOT assay.

The cells (5 × 10) were synchronized and plated in specific medium following the previously described protocol.

Briefly, 2 × 103 cells, HUVEC, TEC, SMCC7721, HCCLM3, MHCC97-H, MHCC97-L, HepG2, and PLC/PRF/5 were plated in the 96-well opaque-walled multiwell plates in culture medium.

2 × 10 HCT 116 s (1.5 × 10 and9 and CCL16 cells) were plated in the seahorse cell plate and incubated overnight.