The metabolite ratio cytidine-5-monophosphate/pentadecanoic acid showed the strongest discrimination between cancer and normal tissues and permitted detection of cancer with a sensitivity of 94.8% and a specificity of 93.9%.
FLAG-tagged Bcl-xL, Bcl-2, Bcl-w and Mcl-1, but not A1 nor CrmA, could detectably co-immunoprecipitate Bax from untreated 293T cells lysed using Triton X-100 (which prompts formation of an active conformation of Bax and permits detection of stable interactions between members of the Bcl-2 subfamily and Bax).
Experiments conducted here demonstrate that tissue that has undergone processing for OPT retains its integrity and immunoreactivity, permitting detection of CD31 and Mac-2 for identification of endothelial cells and macrophages respectively.
In recent years, both serological detection of elevated virus titers and molecular techniques permitting detection of latent EBV within tumor cells have reinforced the role of EBV in the pathogenesis of several malignancies [ 10].
A group size of 10 volunteers per dose was chosen based on the distribution of antibody responses from previous clinical trials [22], [23], and would permit detection of at least a five-fold difference in antibody concentration between dose groups using a Mann-Whitney test, assuming a level of significance of 0.05 and a power of 0.80.
Supplemental breast ultrasound in the population of women with mammographically dense breast tissue (ACR 3 and 4) permits detection of small, otherwise occult, breast cancers.
We also assessed protein-DNA complexes resolved in agarose gels after glutaraldehyde crosslinking, and this permitted detection of RAD-51-ssDNA filaments in the presence of ATP, ADP, or AMP-PNP and in the absence of nucleotide or magnesium ions.
The recently developed specific length amplified fragment sequencing (SLAF-seq) is an efficient strategy that can obtain large numbers of markers with sufficient sequence information to construct high-density genetic maps and permits detection of quantitative trait loci (QTLs) for molecular breeding.
In this study, the estimation of synonymous and nonsynonymous rates permitted detection of the evolutionary patterns between TNL and non-TNL duplicate genes.
We report here that use of this constitutively-active, promiscuous bEBP in DNA microarrays and promoter function assays permitted detection of nearly all known and predicted σ-dependent operons.
