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Before the paint was applied, the linen was first mounted on a temporary stretcher and outlined with a brown border – now visible on the lower border – which was used as a guide to cut the picture down before framing.
Organs fixed over night in 3% formalin/25% sucrose in PBS were frozen down in O.C.T Compound (Tissue-Tek Sakura, Torrance, CA). 10 µm thick cryosections were cut from O.C.T-embedded liver tissue samples and fixed for 10 or 30 min in ice-cold acetone or 4% formalin in PBS, respectively, then washed three times with PBS and outlined with a Pap pen.
These regions are both annotated and outlined with a colored box in Figures 3c.
Sections were mounted on Superfrost Plus slides (Fisher Scientific) and outlined with a delimiting pen (DakoCytomation).
A test zone was determined and outlined with a cellulose disc (5 cm diameter) in a small plastic fly trap.
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(A ) Image shows ASP (highlighted by α-Discs large antibody staining and outlined with dashed white line) from a GRASP experiment in which the fragments of GFP were expressed in the Wg domain of the disc and in the trachea.
Then already the limbus or hem of the colliquament begins to turn purple and is outlined with a tiny line of blood, and almost in its centre there leaps a capering bloody point which is yet so exceedingly small that in its diastole it flashes like the smallest spark of fire, and immediately upon its systole it quite escapes the eye and disappears".
The design is first drawn on the surface of the metal and the motifs outlined with a tracer, which transfers the essential parts of the drawing to the back of the plate.
On a digital fundus photograph, the disk area was outlined with a cursor and then measured, as was the non-perfused area.
On digital photographs of the fundus, the optic disc was outlined with a cursor and then its area was measured, as was also done for the nonperfused area of the retina.
The choice of biologic agent, the indications for its use, the switch between agents, and the considerations of cost are outlined, with a view to guiding the treating physician in managing these cases.
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