Therefore, statistical design techniques should be applied to estimate and optimize the yield loss due to overlay error.
To grow the cells to stationary phase (OD600 = 6 7) and optimize the yield of cIFN-α production in auto-induction system, cultures were incubated at different temperatures (25, 30, and 37°C) and samples were taken at different time points (8, 10, 12, and 24 h).
PM technology has numerous uses in industrial microbiology, particularly in efficiently optimizing the yields and improving the reproducibility of fermentation processes.
The present study is aimed at evaluating and analyzing the effects of the critical process parameters by using response surface modeling method and optimizing the same to enhance the yields of biomass and endospores of a probiotic culture.
Climate change fluctuations accompanying global warming [ 1, 2] are requiring plant breeders to elucidate the molecular mechanisms underlying flowering, and to develop strategies for manipulating and optimizing the flowering times to maximize crop yields.
Optimizing the yield of fresh frozen samples that are well characterized anatomically and diagnostically, and ready for immediate disbursement once categorized, is the quintessence of this protocol.
In order to gain a better understanding of the PDN and to optimize the yield of molecular ions and radicals in the PDN source, a parameter study of the influence of the interelectrode distance on the plasma properties is carried out by means of a discharge model, providing a qualitative as well as a quantitative picture of the plasma.
The use of adaptamer sequences with identical Tm's for the 2nd round of amplification optimized the yields, despite different product lengths and different template concentrations (which were dependent on the yield from the first round reactions).
We found that each set of nucleophiles required a minor variation in the conditions to optimize the yields.
Response surface methodology (RSM) of three factors (ultrasonic power, extraction time and extraction temperature) and three levels was employed to optimize the yield and the antioxidant activity of the polysaccharides.
