Sentence examples for and optimizing the expression from inspiring English sources

To minimize the possibility of selection of the undesired expression-improved mutants, the expression level of wildtype Rubisco was firstly maximized in E. coli through overexpressing the Rubisco-specific molecular chaperone and optimizing the expression conditions.

Several strategies have been developed to overcome such limitations, including expression in plastids [ 41, 42], use of virus-assisted expression systems [ 43- 45], and optimizing the expression cassette to include strong promoters, appropriate 5'- and 3'-UTR, fusing targeting sequences, and conforming codon usage to that of plants [ 40, 46].

As in the presented circuit formed by the TFs GerE and SigK for which an experimental study has provided evidence showing that this FBL plays a key role in enhancing the robustness of the mother cell network and optimizing the expression of target genes [ 58].

With additional background genome selection, the recurrent genome background can further improve the %RGC and optimize the expression of introgressed QTLs.

The plasmids, mouse ESCs (mESCs), expression cassettes and findings that we describe should be of general use in generating other LCA alleles, basal exchange cassettes, and for optimizing the expression of FPs and other reporter alleles in mESCs and mice.

We have successfully expressed and produced gram quantities per liter of active tyrosinase in recombinant E. coli by optimizing the expression conditions and fed-batch cultivation strategy.

In conclusion, we have successfully expressed and produced gram quantities per liter of active tyrosinase in recombinant E. coli by optimizing the expression conditions and fed-batch cultivation strategy.

After optimizing the expression of these chemokine receptors, we proceeded to systematically screen 96 detergents for use in purification procedures.

As a reporter gene for evaluating and optimizing the protocol for transient expression of the introduced gene in planta, we monitored transient expression of β-glucuronidase (GUS) in alfalfa leaves.

Arêde, P., Ministro, J. & Oliveira, D. C. Redefining the role of the β-lactamase locus in methicillin-resistant Staphylococcus aureus: β-lactamase regulators disrupt the MecI-mediated strong repression on mecA and optimize the phenotypic expression of resistance in strains with constitutive mecA expression.

EX1 was initially used to establish and optimize the bacterial expression system by which all the peptides could be eventually individually expressed.