TLF designed and optimized the method, performed mutation screening, and assisted in writing the paper.
M. M. adapted and optimized the method, carried out sequence alignments, primers design and PCR fragments analysis.
HT collected the urine samples, and optimized the methods used.
A ROC curve was plotted for all sets of data to verify the GenoMesh algorithm and to optimize the method of calculating a MeSH-based dissimilarity score based on data in the literature.
We aimed to validate and optimize the measurement method in an in vitro model of the coronary circulation.
In this study, a pGreen 0029 vector containing an eGFP gene under the control of a Ubiquitin promoter and NptII, which is a selective marker gene, was constructed to explore and optimize the electroporation method of C. pyrenoidosa.
To develop and optimize the sonoporation method for TGPAs, we first tested the delivery of fluorescent Texas-red conjugated dextran into native Thermoanaerobacter sp. X514 cells using a Branson B200 Sonifier with fixed ultrasound frequency and power (40 kHz, 19 W).
To further develop and optimize the SIA method, other approaches for combining subalignments can be tested in future studies.
We illustrate the dependency of several parameters on the magnitude of the oxidation peak and try to optimize the method.
