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With additional background genome selection, the recurrent genome background can further improve the %RGC and optimize the expression of introgressed QTLs.
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To minimize the possibility of selection of the undesired expression-improved mutants, the expression level of wildtype Rubisco was firstly maximized in E. coli through overexpressing the Rubisco-specific molecular chaperone and optimizing the expression conditions.
Several strategies have been developed to overcome such limitations, including expression in plastids [ 41, 42], use of virus-assisted expression systems [ 43- 45], and optimizing the expression cassette to include strong promoters, appropriate 5'- and 3'-UTR, fusing targeting sequences, and conforming codon usage to that of plants [ 40, 46].
As in the presented circuit formed by the TFs GerE and SigK for which an experimental study has provided evidence showing that this FBL plays a key role in enhancing the robustness of the mother cell network and optimizing the expression of target genes [ 58].
EX1 was initially used to establish and optimize the bacterial expression system by which all the peptides could be eventually individually expressed.
Herein, we explored the expression of A20L tagged with an Ub-tag and SUMO1/2/3/4-tags SUMO1/2/3/4-tagsexpression approach of AMP productooptimize
To provide a clear picture on this issue, we present in this study a rational and systematic approach to optimize the expression of a biocatalyst in a reproducible fashion.
In the current report, we conducted a pilot antigen engineering study to optimize the expression and immunogenicity of subunit VP1 antigen for the design of EV71 vaccines.
Surface enhanced laser desorption/ionization-mass spectrometry (SELDI-MS) was evaluated to optimize the expression and to develop the purification of a recombinant mouse protein: a transmembrane adaptor involved in T cell receptor signaling named "linker for activation of T cells" (LAT).
A series of modifications to optimize the expression of those enzymes and the medium have been done.
Suppression by miR-31 should optimize the expression of RhoA involved in cytoskeletal organization and following bone resorption.
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