The phrase "and normalisation of data" is correct and usable in written English.
It can be used in contexts related to data management, database design, or data analysis, where the process of organizing data to reduce redundancy and improve integrity is discussed.
Example: "The project involves data cleaning, transformation, and normalisation of data to ensure accuracy and consistency."
Alternatives: "and data normalization" or "and data structuring".
Hybridisation conditions, dye-label properties, RNA extraction process etc. are such sources of variation, and normalisation of data adjusts for these and is a required step.
RNA isolation, cDNA synthesis, quality control checks and normalisation of data on a set of three reference genes (HPRT1, HBMS and B2M) were carried out as previously described (Sieuwerts et al, 2005; Meijer et al, 2008).
This resulted in resolution of headaches, prevention of impending wound breakdown and normalisation of ICP data.
Quality control and normalisation of methylation data was undertaken using the analysis pipeline reported elsewhere [ 31].
JUR performed condensation and normalisation of the microarray data, the N-way ANOVAs as well as the GO- and promoter analyses.
Raw data from each individual channel of each array was also subjected to principle components analysis (PCA) and hierarchical clustering following normalisation of data (log2, mean set to 0 and standard deviation set to 1) using Partek software.
It should be noted that throughout the study, following densitometric analysis and normalisation of the Western blot data, control values were taken to represent 100% and an average increase or decrease greater than 20% after experimental treatment from triplicate samples was considered to represent changes in expression or activity of the protein of interest.
This ratio allows normalisation of data and elimination of variability due to cartilage quality, cartilage wet weight, and experimental variation.
However, proper and highly reliable reference genes are needed for normalisation of data, as normalisation by total pathogen RNA in mixed host-pathogen samples is usually not possible.
According to those data and normalisation of expression levels with housekeeping genes (Actc, Gapdh, Rps29, B2m, Ppia, Gusb, Tbp) Rsad2 and Colec11 transcript amounts are 2.78 and 1.93 times higher in B6 than in D2 mice, respectively, under standard diet conditions [ 42] when D2 animals are heavier than B6 animals.
Reporter gene constructs (1 μg) and internal control TK renilla construct (10 ng) used for normalisation of data, were co transfected using TurboFect (Thermo Scientific) following manufacturers' instructions.
