Sentence examples for and mutants of the from inspiring English sources

We now determined crystal structures of DARPins with one or three full-consensus internal repeats (NI1C or NI3C) between an N-terminal capping repeat and mutants of the C-cap.

These results suggest that wild-type PKM2 and mutants of the PKM2 tetramer tend to adopt the T-state conformation in the apo form but prefer an R-state conformation in the presence of either FBP or an analog of PEP.

This design can be generalized to study many other protein activities, using a single, genetically encoded chain incorporating the protein to be studied, an "affinity reagent" which binds only to the activated form of the targeted protein, and mutants of the green fluorescent protein (GFP) that undergo fluorescence resonance energy transfer (FRET).

Examples of these immunogens are LVS, attenuated Francisella novicida strains and mutants of the virulent SchuS4 strain [7] [10].

The wild type and mutants of the PDE9A2 catalytic domain were purified by using the similar protocols previously reported [24].

The ligand overlay assay was performed by spotting the purified wild type protein and mutants of the collagen binding M-protein FOG (5 µg) [5] on nitrocellulose.

Gel filtration of wild-type and mutants of PKM2 in the absence (D) or presence (E) of FBP.

We first measured the enzymatic activities for wild-type and mutants of PKM2 and calculated the Km values for PEP.

The optimum temperature for the peroxidase activity of the WT and mutants of CYP175A1 was ~ 70 °C.

The fis and hns mutants of the LZ41 and LZ54 strains were obtained by phage P1 transduction.

The same was true of a series of truncation and alanine mutants of the SmD3 and SmB subunits of the yeast Sm ring (Schwer and Shuman 2015).