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Fluorescence quantification and morphometric analysis were performed using MetaMorph software (Universal Imaging).
Image processing and morphometric analysis were performed using the Zeiss LSM software, ImageJ (http://rsbweb.nih.gov/ij) and Matlab (version 7, MathWorks, Ismaning, Germany).
Electron microscopy and morphometric analysis were performed using a fluorescence microscope (Olympus, Tokyo, Japan).
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Images were captured using Nikon Eclipse 80i and morphometric analysis was performed using NIS Elements Imaging Software Nikon Instruments Inc ... Circumference measurements of the external elastic lamina (EEL) and the internal elastic lamina (IEL) were used to calculate the area and vessel thickness.
Images were captured on a Zeiss AxioImagerZ1 microscope, and morphometric analysis was performed using MetaMorph7.5 (Molecular Devices).
Images were obtained with a Nikon E-800 fluorescence microscope, 400 times magnification, and morphometric analysis was performed using the software Image ProPlus 4.5.
The morphometric analysis was performed using the Image-Pro Discovery software (Media Cybernetics, Silver Spring, MD, USA).
Morphometric analysis was performed using a MetaSystems image analysis system.
Morphometric analysis was performed using ImageJ in 15 20 different micrographs for each condition after thresholding.
A morphometric analysis was performed using an ImageTool software 3.00 version (University of Texas Health Science Center in San Antonio, USA).
The morphometric analysis was performed using OPTIMAS™ (Bioscan Incorporated, Edmonds, WA, USA) software.
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