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The most rapid method of separation was published by Hall et al. [ 21] for TIMS and modified at the Geological Survey of Israel for MC-ICP-MS.
For BVAB3, a combination of forward and reverse primers published in two different studies were used and modified at the 5′-end (see Additional file 1: Table S1).
The fluorogenic probes were labeled at the 5'end with the fluorescent reporter dye FAM (6-carboxy-fluorescein) and modified at the 3'end with the quencher dye TAMRA (6-carboxy-tetramethylrhodamine).
In this study, IS117 was derived from a linearized and amplified copy in the Streptomyces coelicolor A3(2) chromosome and modified at the ends for integration into the chromosome of A. pretiosum.
Wild type and mutant allele capture oligonucleotide probes (Table 1) were synthesized and modified at the 5' terminus (TAG Copenhagen, Denmark) and coupled to carboxylated microspheres as described by Luminex.
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Asiatic acid (AA) derivatives 4 and 5 modified at the C-11 and C-28 positions were designed and synthesized, their structures were confirmed using HRMS, 1H NMR and 13C NMR.
The Prevotella spp. primers were previously published and slightly modified at the 5′-end (see Additional file 1: Table S1).
Computer-assisted mammographic density assessment was performed using a method that was first developed in Toronto [ 15] and later modified at the University of Southern California in Los Angeles [ 16].
The biogenesis of spliceosomal snRNPs takes place in both the cytoplasm where Sm core proteins are added and snRNAs are modified at the 5' and 3' termini and in the nucleus where snRNP-specific proteins associate.
The unsteady flows over a shallow rectangular cavity at Mach 1·5 and 2·5 are modified at the leading edge by using compression ramps, expansion surfaces, and mass injection.
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